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EFFECT OF SORO OIL ON KERATINOCYTE PROLIFERATION BY IN
VITRO AND IN VIVO METHODS
SORO Oil, studied in detail for its effect on
keratinocyte proliferation suppression. The study was
carried out in The Department of Microbiology, The New
College, Chennai.
For in vitro study, EPISKIN & EpiDerm model were used.
The fast growing cell line were maintained either
cryo-preserved or in 12% CO2 chamber. The SORO oil was
prepared in various dilutions in RPMI 1640 medium.
Immunomodulatory property of SORO Oil
SORO Oil was studied for its immunomodulatory property
both by in vitro and in vivo techniques. The
phagocytes were isolated from the peritoneal cavity of
Balp C mice after injecting with thyoglycolate. The
phagocytes were washed and fixed on a flat bottom
ELISA plate and were maintained in RPMI 1640. The
adhered phagocytes were pre-treated with different
dilutions of SORO Oil for 20 minutes. Later the cells
were infected with opsonized fungal cells of Candida
albicans. The level of phagocytosis and phagocytic
killing (phagocytic Index, PI) were calculated by the
following formula PI = Test / contro l Similarly,
SORO Oil was administered orally to mice after
injecting with SRBC (Sheep Red Blood Cells) and the
level of antibody production was estimated by
haemaglutination.
Immunomodultory studies with SORO Oil reveal that SORO
Oil can significantly increase both phagocyte and
antibody mediated immunity.
% reduction of cell division after treated with SORO
Oil atVarious concentrations
Dose dependant decrease in cell multiplication was
observed in the in vitro study. 20mg/ml concentration
of SORO Oil was very effective in retarding the cell
division to 90% level.
SORO Oil at 50 mg/ml and above produced necrosis in
the cell line.
The kinetics of cell division in the case of treated
vs. untreated cells was 2:8. The nuclear spindle
formation is arrested during the treatment of cells
with SORO Oil. This phenomenon was studied by mitosis
inhibition assay using onion root tip assay. SORO Oil
arrest the cell division especially during the mid
anaphase to metaphase stage.
Clinical and experimental studies on the efficacy of
SORO Oil a herbal preparation - CCRAS
The efficacy of SORO Oil was confirmed on 300 cases
taken up for trials in the out patient department of
Central Research Institute, Siddha at Chennai, India
between 1998 to2001 The results were published in the
Monograph by Central Council for Research in Ayurveda
and Siddha (CCRAS), Ministry of Health and Family
Welfare, Government of India, New Delhi .
The results together with the follow-up study
indicated SORO Oil proved to be an ideal drug that
provided marked relief from clinical symptoms, broke
the cycle of recurrence, minimizes extent of
complications and prevents complications completely.
Long-term application did not invite or foresee any
hazards, unlike steroidal formulations.
Papers presented in Domestic conferences by Dr.
Gaurang Joshi
Dr. Gaurang Joshi and his associates have presented a
number of research and clinical study papers in
Domestic and International forums.
30 patients were put to treatment with SORO Oil for a
period of 12 weeks. In a majority of the cases, the
clinical symptoms started receding within the period
of study. SORO Oil was found to a great extent, an
ideal drug for the treatment of Psoriasis. It was
found to be clinically effective, Non-toxic,
cosmetically acceptable and economic. The findings
results of the above study done by Prof. Dr. J.M.
Boopalraj, Dr. Frederick Manuel et al. were presented
at the 23rd National Conference of IADV&L (Indian
Association of Dermatologists, Venerologists and
Leprologists) in 1998 at Chennai and later published
in the issue of "The Antiseptic" Medical Journal.
A paper titled " Treatment of psoriasis with Wrightia
tinctoria leaf extract" was presented by Dr. Frederick
Manuel at the above conference in . The paper
highlighted that the extracts of the leaves of the
plant are effective and inexpensive for the treatment
of psoriasis and that most patients had long
remissions on follow-up. The paper was presented on
the basis on the basis of the trials done at the
Kilpauk Medical College, Chennai.
Use experience reports of practising Dermatologists.
SORO Oil and SORO Ointment have been drugs of choice
for many practising Dermatologists in India and under
their care, more and more patients are benefiting.
Their observations have been encouraging. There are
many more Dermatologists, General Physicians and
practitioners of alternative medicines who share the
observation. A few are presented below:
"An ideal drug for the treatment of Psoriasis should
have all the following characteristics- Effectiveness,
Relative non-toxicity, Cosmetic applicability, and
Cheapness (economical) . In my experience I have found
that SORO Oil satisfies all these parameters to a
great extent"
" The more chronic the lesion the more striking is the
improvement with the application of SORO Oil"
"SORO Oil can be considered as one of the drugs of
choice for the treatment, control and overall
management of Psoriasis"
" I have personally prescribed SORO Oil to some of my
eligible patients and I found the results encouraging.
Most of my patients got good remission"
" I would like to thank Dr. Gaurang Joshi for
introducing SORO Oil on scientific lines in the
treatment of Psoriasis and conclude that SORO Oil is
really a breakthrough in the management of all types
of Psoriasis"
"For the last few years I have been trying SORO Oil
for my patients and I find the results are very
effective in regard to several signs and symptoms
affecting patients. Most of my patients had no
remissions for a long period. None of my patients
complained of any side effects"
" SORO Oil is an ideal drug for psoriasis and has no
side effects"
" I have been prescribing SORO Oil and SORO Ointment
to my Psoriasis patients and some cases of Seborrhic
dermatitis with satisfactory results. SORO Ointment
gives good results for Pityriasis Versicolor."
This also explains the modern views where scientific
community believes that there is no proven link
between diet and its effect on psoriatic conditions
but still some patients experience remarkable
improvements in their psoriatic conditions with the
control of some of the diets and then the same diet
control does not produce any effect on other patients
and yet the same diet control does not produce any
significant effect on same patient after some time.
How can we explain this anomaly.
Our special Dietary Guideline which we provide to
every psoriatic patient under our treatment is based
upon above Ayurvedic View and help every psoriatic
patients in their early cure.
References:
E. Noiesen, M.D. Munk, K. Larsen, M.
Høyen and T. Agner. (2007) Use of complementary
and alternative treatment for allergic contact
dermatitis. British Journal of Dermatology 157:2,
301-305
Eran Ben-Arye, M. Frenkel, M. Ziv. (2004) An
Approach to Teaching Dermatologists About
Complementary Medicine. The Journal of Alternative and
Complementary Medicine 10:5, 899
E. Ernst, MD, PhD, FRCP. (2003) CAM in
dermatology: Telling fact from fiction. International
Journal of Dermatology 42:12, 979-980
Gottlieb, AB, Lifshitz, B, Fu, SM,
Staiano-Coico, L, Wang, CY, Carter, DM: Expression of
HLA-DR molecules by keratinocytes and presence of
Langerhans cells in the dermal infiltrate of active
psoriatic plaques. J Exp Med 1986 164: 1013-1028,
Gottlieb, AB: Immunologic mechanisms in
psoriasis. J Am Acad Dermatol 1988 18: 1376-1380, |
Gottlieb, AB, Chang, CK, Posnett, DN,
Fanelli, B, Tam, JP: Detection of transforming growth
factor alpha in normal, malignant, and
hyperproliferative human keratinocytes. J Exp Med 1988
167: 670-675, Grossman, RM, Krueger, J, Yourish, D,
Granelli-Piperno, A, Murphy, DP, May, LT, Kupper, TS,
Sehgal, PB, Gottlieb, AB: Interleukin-6 (IL-6) is
expressed in high levels in psoriatic skin and
stimulates proliferation of cultured human
keratinocytes. Proc Natl Acad Sci USA 1989 86:
6367-6371, Krueger, GG: Psoriasis: current concepts of
its etiology and pathogenesis. In: Dobson RL (ed.).
Year Book of Dermatology 1981, Year Book Medical
Publishers, Chicago, pp 33-70,
Baker, BS, Swain, AF, Fry, L, Valdimarsson,
H: Epidermal T lymphocytes and HLA-DR expression in
psoriasis. Br J Dermatol 1984 11: 555-564,
Baadsgaard, O, Tong, P, Hansen, E, Taylor,
RS, Chan, L, Mannie, A, Wantzin, GL, Voorhees, JJ,
Fox, D, Cooper, KD: Lesional psoriasis T cell clones
express UM4D4, the surface component of an antigen
independent T cell activation pathway (abstr). J
Invest Dermatol 1988 90: 545,
Kaplan, G, Witmer, MD, Nath, I, Steinman, RM,
Laal, S, Prasad, HK, Sarno, EN, Elvers, U, Cohn, ZA:
Influence of delayed immune reactions on human
epidermal keratinocyres. Proc Natl Acad Sci USA 1986
183: 3469-3473,
Nathan, CF, Kaplan, G, Levis, WR, Nusrat, A,
Wirmer, MD, Sherwin, SA, Job, CK, Horowitz, CR,
Steinman, RM, Cohn, ZA: Local and systemic effects of
intradermal recombinant interferon-gamma in patients
with lepromatous leprosy. N Engl J Med 1986 315: 6-15.
For More Details contact
Dr.Gaurang Joshi
B.A.M.S.(Gold Medalist)
Ayurveda Immunologist,Panchakarma Physician,
Skin Specialist,
2,Paras Society,
Nirmala Convent Road,
Opp:Physiotherapy College,
RAJKOT.360001.
Gujarat.
India.
Email:drgaurang_joshi@yahoo.com
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